1DHS
CRYSTAL STRUCTURE OF THE NAD COMPLEX OF HUMAN DEOXYHYPUSINE SYNTHASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 108.300, 108.300, 69.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.153 |
| Rwork | 0.153 |
| R-free | 0.24200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.802 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | 0.253 |
| Total number of observations | 108771 * | |
| Number of reflections | 18479 | |
| <I/σ(I)> | 21.2 | 3.1 |
| Completeness [%] | 86.1 | 41.9 |
| Redundancy | 5.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 20 * | 9.0 MG/ML PROTEIN IN 1.7M PHOSPHATE FINAL PH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.50 (mg/ml) | |
| 2 | 1 | drop | NAD | 0.05 (M) | |
| 3 | 1 | drop | 1,7-diaminoheptane | 0.05 (M) | |
| 4 | 1 | drop | sodium potassium phosphate | 0.85 (M) | |
| 5 | 1 | drop | HEPES | 50 (mM) | |
| 6 | 1 | reservoir | sodium potassium phosphate | 1.7 (M) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
