1DHN

1.65 ANGSTROM RESOLUTION STRUCTURE OF 7,8-DIHYDRONEOPTERIN ALDOLASE FROM STAPHYLOCOCCUS AUREUS

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-09-04
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	61.300, 61.300, 124.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.197
Rwork	0.197
R-free	0.25200
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.930
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.650
Rmerge	0.047
Total number of observations	56369 *
Number of reflections	14310
<I/σ(I)>	12.4
Completeness [%]	98.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	magnesium formate	200 (mM)
2	1	reservoir	magnesium formate	175 (mM)