1DGL

LECTIN FROM DIOCLEA GRANDIFLORA COMPLEXED TO TRIMANNOSIDE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1995-06
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	92.110, 92.110, 202.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600 *
R-factor	0.182 *
Rwork	0.189
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cna
RMSD bond length	0.010
RMSD bond angle	26.250 *
Data reduction software	SADIE
Data scaling software	SADIE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.500
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.064 *	0.214 *
Number of reflections	26679 *	4349 *
<I/σ(I)>	13	2.6
Completeness [%]	74.0	70.5 *
Redundancy	3.9 *	3.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	4 *	pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	sugar	5 (mM)
3	1	reservoir		0.6 (M)
4	1	reservoir	HEPES	50 (mM)
5	1	reservoir		5 (mM)