1DGJ
CRYSTAL STRUCTURE OF THE ALDEHYDE OXIDOREDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 156.639, 156.639, 177.149 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.800 - 2.800 |
| R-factor | 0.164 * |
| Rwork | 0.164 |
| R-free | 0.22400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.810 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.800 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.169 | 0.561 |
| Total number of observations | 190328 * | |
| Number of reflections | 31573 * | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 98.2 | 98 |
| Redundancy | 10 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | ammonium sulphate, MES, dioxane, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | reservoir | dioxane | 10 (%(v/v)) | |
| 4 | 1 | drop | protein | 15 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) |
