1DGC
THE X-RAY STRUCTURE OF THE GCN4-BZIP BOUND TO ATF/CREB SITE DNA SHOWS THE COMPLEX DEPENDS ON DNA FLEXIBILITY
Experimental procedure
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.660, 58.660, 86.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.860 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.090 * |
| Completeness [%] | 99.0 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NA CITRATE | ||
| 4 | 1 | 1 | CACL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 6000 | ||
| 7 | 1 | 2 | NA CITRATE | ||
| 8 | 1 | 2 | CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2-0.4 (mM) | |
| 2 | 1 | drop | oligonucleotide | ||
| 3 | 1 | drop | sodium citrate | 15 (mM) | |
| 4 | 1 | drop | 15 (mM) | ||
| 5 | 1 | drop | PEG6000 | 9 (%(w/v)) | |
| 6 | 1 | reservoir | sodium citrate | 30 (mM) | |
| 7 | 1 | reservoir | 30 (mM) | ||
| 8 | 1 | reservoir | PEG6000 | 18 (%) |
