1DG3

STRUCTURE OF HUMAN GUANYLATE BINDING PROTEIN-1 IN NUCLEOTIDE FREE FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-07-20
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	155.470, 41.030, 121.000
Unit cell angles	90.00, 109.42, 90.00

Refinement procedure

Resolution	29.940 - 1.800
R-factor	0.242
Rwork	0.242
R-free	0.27600
RMSD bond length	0.006
RMSD bond angle	19.600 *
Data scaling software	XDS
Phasing software	CCP4
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.299
Number of reflections	66520
<I/σ(I)>	17.06
Completeness [%]	98.4	95.1
Redundancy	5.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	20 *	100MM MES-NAOH, 7.5% PEG6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG6000	5 (%)
3	1	reservoir	Mes-NaOH	100 (mM)