1DFO

CRYSTAL STRUCTURE AT 2.4 ANGSTROM RESOLUTION OF E. COLI SERINE HYDROXYMETHYLTRANSFERASE IN COMPLEX WITH GLYCINE AND 5-FORMYL TETRAHYDROFOLATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-06-01
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	77.460, 172.240, 95.050
Unit cell angles	90.00, 104.12, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.174
Rwork	0.174
R-free	0.19600
RMSD bond length	0.006
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.000	2.490
High resolution limit [Å]	2.400	2.410
Rmerge	0.230	1.000
Number of reflections	83968 *
<I/σ(I)>	7.1
Completeness [%]	89.0 *	78 *
Redundancy	4.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2	293	Stover, P., (1993) J.Mol.Biol., 230, 1094. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	25 (%sat)
2	1	drop	enzyme	30-50 (mg/ml)
3	1	reservoir	potassium phosphate	40 (%sat)
4	1	reservoir	PEG400		or glycerol or 1,2,3-heptanetriol