1DF4
INTERACTIONS BETWEEN HIV-1 GP41 CORE AND DETERGENTS AND THEIR IMPLICATIONS FOR MEMBRANE FUSION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 52.383, 52.383, 60.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.500 - 1.450 |
| Rwork | 0.200 |
| R-free | 0.24500 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 36.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.500 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.028 | 0.125 |
| Total number of observations | 43163 * | |
| Number of reflections | 11818 | |
| <I/σ(I)> | 22.5 | |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | SODIUM CITRATE, AMMONIUM DIHYDROGEN PHOSPHATE, BETA-OG, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium dihydrogen phosphate | 1.0 (M) | |
| 4 | 1 | reservoir | beta OG | 35 (mM) |
