1DEW
CRYSTAL STRUCTURE OF HUMAN APE1 BOUND TO ABASIC DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.221, 72.249, 93.749 |
| Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.1951 |
| Rwork | 0.195 |
| R-free | 0.28600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.085 | 0.367 |
| Total number of observations | 61212 * | |
| Number of reflections | 24575 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 93.0 | 91.6 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 298 | MPEG 2000, LITHIUM SULFATE, CACODYLATE BUFFER, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | LI2SO4 | ||
| 2 | 1 | 1 | CACODYLATE BUFFER | ||
| 3 | 1 | 1 | MPEG 2000 | ||
| 4 | 1 | 2 | MPEG 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | cacodylate | 100 (mM) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | mPEG2000 | 27 (%) |
