1DEO

RHAMNOGALACTURONAN ACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.55 A RESOLUTION WITH SO4 IN THE ACTIVE SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1995-04-25
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	52.140, 56.870, 71.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.164 *
Rwork	0.164
R-free	0.20000
RMSD bond length	0.014
RMSD bond angle	24.300 *
Data reduction software	ROTAVATA
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.035	0.293
Number of reflections	28693
<I/σ(I)>	13.7
Completeness [%]	91.0	77.9
Redundancy	7.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5	298	Lithium sulfate, Na acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		1.4 (M)
2	1	1	sodium acetate	0.1 (M)
3	1	1	protein	40 (OD280)