1DE5

L-RHAMNOSE ISOMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	195
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	169.442, 162.693, 77.022
Unit cell angles	90.00, 110.68, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.156
Rwork	0.156
R-free	0.27500
RMSD bond length	0.015 *
RMSD bond angle	2.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.080
Number of reflections	85683
<I/σ(I)>	9.7
Completeness [%]	86.0	88
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	0.2 M CITRATE, 0.1-M HEPES, 22.5 - 30.0 % PEG 8000, 10 % ISOPROPANOL, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop		100 (mM)
4	1	reservoir	citrate	0.2 (M)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir	PEG8000	22.5-30.0 (%(w/w))
7	1	reservoir	isopropanol	10 (%(w/w))