1DDV

CRYSTAL STRUCTURE OF THE HOMER EVH1 DOMAIN WITH BOUND MGLUR PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1999-10-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	55.700, 48.600, 35.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.241 *
Rwork	0.242
R-free	0.28900 *
RMSD bond length	0.019 *
RMSD bond angle	1.924 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.088	0.377
Number of reflections	7996
<I/σ(I)>	28
Completeness [%]	98.3	87
Redundancy	7.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 3350, SODIUM ACETATE, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	peptide	10 (mg/ml)
3	1	reservoir	PEG3350	30 (%)
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	Tris	100 (mM)