1DDN
DIPHTHERIA TOX REPRESSOR (C102D MUTANT)/TOX DNA OPERATOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 1996-02-15 |
| Detector | BRANDEIS |
| Spacegroup name | P 41 |
| Unit cell lengths | 117.050, 117.050, 145.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.24 * |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.096 |
| Total number of observations | 107910 * |
| Number of reflections | 34176 * |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 87.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA mixture | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 6-12 (%) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | MES | 100 (mM) |
