1DD1

CRYSTAL STRUCTURE ANALYSIS OF THE SMAD4 ACTIVE FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	1999-06-16
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	142.433, 142.433, 195.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.620
R-factor	0.218
Rwork	0.218
R-free	0.17500
RMSD bond length	0.006
RMSD bond angle	1.207
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.680
High resolution limit [Å]	2.620	2.620
Rmerge	0.143	0.020
Number of reflections	29751
<I/σ(I)>	11.17
Completeness [%]	92.9	96.3
Redundancy	5.02	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		drop was combined with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	PEG4000	10 (%)
4	1	reservoir		200 (mM)