1DCW
STRUCTURE OF A FOUR-WAY JUNCTION IN AN INVERTED REPEAT SEQUENCE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1999-10-08 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.500, 23.500, 76.900 |
| Unit cell angles | 90.00, 114.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.31700 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.160 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.218 |
| Total number of observations | 18370 * | |
| Number of reflections | 6322 | |
| <I/σ(I)> | 15.6 | 2.7 |
| Completeness [%] | 97.4 | 96.7 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | MPD, CACL2, SODIUM CACODYLATE, pH 7.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CACL2 | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | 0.25 (mM) | ||
| 2 | 1 | sodium cacodylate | 75 (mM) | ||
| 4 | 1 | 1 | 2.5 (%) | ||
| 5 | 1 | 2 | MPD | 30 (%(v/v)) |
