1DCV
B-DNA DECAMER WITH CENTRAL TA DINUCLEOTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.100, 25.900, 39.900 |
| Unit cell angles | 90.00, 122.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IDEAL B-DNA |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data scaling software | d*TREK |
| Phasing software | EPMR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.810 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.052 * | 0.418 |
| Total number of observations | 7532 * | |
| Number of reflections | 2009 | |
| <I/σ(I)> | 12.8 | 3.1 |
| Completeness [%] | 98.2 | 91.6 |
| Redundancy | 3.7 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | DNA | 0.5 (mM) | |
| 2 | 1 | 1 | sodium cacodylate | 20 (mM) | |
| 3 | 1 | 1 | 50 (mM) | ||
| 4 | 1 | 1 | PEG200 | 10 (%) | |
| 5 | 1 | 2 | PEG200 | 30 (%(v/v)) |
