1DCF

CRYSTAL STRUCTURE OF THE RECEIVER DOMAIN OF THE ETHYLENE RECEPTOR OF ARABIDOPSIS THALIANA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-12-05
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	47.920, 47.920, 111.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.500
R-factor	0.227
Rwork	0.222
R-free	0.27090
RMSD bond length	0.006
RMSD bond angle	1.433
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.049	0.423
Number of reflections	4410
<I/σ(I)>	13.2
Completeness [%]	87.0	93.5
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	277	Li2SO4, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22 (mg/ml)
2	1	reservoir		1.6 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir		0.03 (%)