1DC1
RESTRICTION ENZYME BSOBI/DNA COMPLEX STRUCTURE: ENCIRCLEMENT OF THE DNA AND HISTIDINE-CATALYZED HYDROLYSIS WITHIN A CANONICAL RESTRICTION ENZYME FOLD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.294, 87.823, 99.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.25300 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 | 0.519 |
| Number of reflections | 77509 | |
| <I/σ(I)> | 24.5 | 2.1 |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | EQUILIBRATION AGAINST RESERVOIR OF 20% (V/V) DIOXANE, 2% (V/V) ETHYLENE GLYCOL AND 5 MM DTT. DROPS WERE FORMED BY MIXING 2.5 UL PROTEIN-DNA COMPLEX (10 MG/ML PROTEIN; 1.1 FOLD MOLAR EXCESS DNA) IN BUFFER (20 MM TRIS-HCL PH 7.6, 300 MM NACL, 0.1 MM EDTA, 1 MM DTT, 0.02% NA AZIDE) WITH 2.5 UL DISTILLED WATER, 2.5 UL RESERVOIR SOLUTION AND 1.5 UL 350 MM N-HEPTYL-B-D-GLUCOSIDE, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS HCL | ||
| 10 | 1 | 2 | DIOXANE | ||
| 11 | 1 | 2 | ETHYLENE GLYCOL | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | EDTA | ||
| 4 | 1 | 1 | N-HEPTYL-B-D-GLUCOSIDE | ||
| 5 | 1 | 1 | NAN3 | ||
| 6 | 1 | 1 | DTT | ||
| 7 | 1 | 1 | DIOXANE | ||
| 8 | 1 | 1 | ETHYLENE GLYCOL | ||
| 9 | 1 | 2 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.3 (mg/ml) | |
| 2 | 1 | reservoir | p-dioxane | 20 (%(v/v)) | |
| 3 | 1 | reservoir | ethylene glycol | 2 (%(v/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | n-heptyl-beta-D-glucoside | 350 (mM) |
