1DBP

IDENTICAL MUTATIONS AT CORRESPONDING POSITIONS IN TWO HOMOLOGOUS PROTEINS WITH NON-IDENTICAL EFFECTS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	74.840, 88.880, 39.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 2.200
R-factor	0.16
Rwork	0.160
RMSD bond length	0.008
RMSD bond angle	1.070
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.070 *
Total number of observations	41653 *
Number of reflections	12590 *
Completeness [%]	90.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		used to seed *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	32 (%(w/v))
2	1	drop	sodium phosphate	90 (mM)
3	1	drop	ribose	8 (mM)
4	1	reservoir	PEG4000	26 (%)