1DBO
CRYSTAL STRUCTURE OF CHONDROITINASE B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.600, 74.400, 58.700 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.700 |
| R-factor | 0.181 * |
| Rwork | 0.181 |
| R-free | 0.21700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.750 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.129 |
| Total number of observations | 135533 * | |
| Number of reflections | 44142 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 92.2 | 57.8 |
| Redundancy | 4.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.7 | 292 * | Li, Y., (1999) Acta Crystallog. Sect., D55, 1055. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-8 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 19 (%(w/v)) | |
| 3 | 1 | reservoir | Bicine | 100 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | ammonium acetate | 0.15 (M) | |
| 6 | 1 | reservoir | MPD | 15 (%(v/v)) |
