1DBN
MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.742, 88.450, 84.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.25300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.028 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.109 | 0.442 |
| Total number of observations | 171599 * | |
| Number of reflections | 16436 * | 1618 * |
| <I/σ(I)> | 14.49 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 20 * | PEG 1000, PEG 8000, sialyllactose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | siallylactose | 5-molar excess | |
| 3 | 1 | drop | PEG1000 | 9 (%(w/v)) | precipitant |
| 4 | 1 | drop | PEG8000 | 9 (%(w/v)) | precipitant |
