1DBI
CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-19 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.050, 51.680, 52.780 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.182 |
| R-free | 0.24700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.372 |
| Total number of observations | 97032 * | |
| Number of reflections | 20847 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 87.4 | 47 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | PEG 4000, acetate, calcium chloride, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | TES | 10 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | Triton X-100 | 0.01 (%) | |
| 5 | 1 | reservoir | PEG4000 | 14 (%) | |
| 6 | 1 | reservoir | acetate | 100 (mM) |
