1DAR

ELONGATION FACTOR G IN COMPLEX WITH GDP

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	76.300, 106.400, 115.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.22
Rwork	0.220
R-free	0.29000
RMSD bond length	0.014
RMSD bond angle	26.290 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALEIT))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	0.260
Total number of observations	284145 *
Number of reflections	32595
<I/σ(I)>		2.9
Completeness [%]	87.0	46
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *	Reshetnikova, L. S., (1983) FEBS Lett., 154, 149. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	TEF-G	7-10 (mg/ml)
2	1	drop	imidazole-HCl	20 (mM)
3	1	drop	sodium azide	3 (mM)
4	1	reservoir	MPD	20 (%(v/v))