1DAG
DETHIOBIOTIN SYNTHETASE COMPLEXED WITH 7-(CARBOXYAMINO)-8-AMINO-NONANOIC ACID AND 5'-ADENOSYL-METHYLENE-TRIPHOSPHATE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-02 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.200, 49.200, 61.800 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.640 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 24.500 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.640 * |
| Rmerge | 0.059 |
| Total number of observations | 61271 * |
| Number of reflections | 22267 |
| Completeness [%] | 87.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | cocrystallization * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | AMPPCP | 5 (mM) | |
| 2 | 1 | 1 | 200 (mM) | or 100mM MnCl2 |
