1DAA

CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	58.500, 76.200, 73.300
Unit cell angles	90.00, 103.90, 90.00

Refinement procedure

Resolution	10.000 - 1.940
R-factor	0.184
Rwork	0.184
RMSD bond length	0.013
RMSD bond angle	24.400 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.940
Rmerge	0.063
Total number of observations	106545 *
Number of reflections	40269
Completeness [%]	88.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *		Stoddard, B., (1987) J. Mol. Biol., 196, 441. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	pyridoxal phosphate	0.050 (mM)
2	1	reservoir	MPD	1 (%(v/v))
3	1	reservoir	ammonium sulfate	29 (%)
4	1	drop	protein	6.7 (mg/ml)
5	1	drop	pyridoxal phosphate	0.050 (mM)
6	1	drop	MPD	1 (%(v/v))
7	1	drop	ammonium sulfate	29 (%)