1DA2
MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO4CG): N4-METHOXYCYTOSINE/GUANINE BASE-PAIRS IN Z-DNA
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P21 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.170, 30.360, 43.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.181 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.039 * |
| Number of reflections | 2944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | (mM) | |
| 2 | 1 | drop | sodium cacodylate | 24 (mM) | |
| 3 | 1 | drop | 12 (mM) | ||
| 4 | 1 | drop | spermine | 4.7 (mM) | |
| 5 | 1 | reservoir | MPD | 40 (%) |
