1DA1
STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P21 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.940, 30.850, 49.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.250 |
| R-factor | 0.156 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 1105 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 * | 4-18 * | pH 8.20, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 5 | 1 | 1 | NA ACETATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | ISOPROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | (mM) | |
| 2 | 1 | drop | magnesium chloride | 20 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 4 | 1 | drop | Tris hydrochloride | 50 (mM) | |
| 5 | 1 | drop | sodium acetate | 25 (mM) | |
| 6 | 1 | reservoir | isopropanol | 5 (%) | in water |
