1D9X
CRYSTAL STRUCTURE OF THE DNA REPAIR PROTEIN UVRB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1999-09-07 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.207, 150.207, 79.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.256 * |
| Rwork | 0.256 |
| R-free | 0.32400 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.032 |
| Data reduction software | MARMAD |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.550 |
| Number of reflections | 32030 | |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 8 mg/ml UvrB, 500 mM NaCl, 14-18% PEG 6000, 10 mM ZnCl2, 100 mM Bicine pH 9, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 10 | 1 | reservoir | PEG6000 | 20 (%) | |
| 11 | 1 | reservoir | 500 (mM) | ||
| 12 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.1 (mM) | |
| 6 | 1 | drop | dodecylmaltoside | 0.03 (%) | |
| 7 | 1 | drop | PEG6000 | 14-18 (%) | or PEG20000 |
| 8 | 1 | drop | 10 (mM) | ||
| 9 | 1 | drop | Bicine | 100 (mM) |
