1D9W
BACTERIOPHAGE T4 LYSOZYME MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.890, 60.890, 97.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 1.910 |
| Rwork | 0.179 |
| RMSD bond length | 2.982 |
| RMSD bond angle | 0.024 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT (V. 5-E) |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | |
| Number of reflections | 15172 | |
| Completeness [%] | 89.1 | 54.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2.1-M phosphate, 10 mM BME, 20 mM oxBME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
