1D9V

HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1997-01-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	106.746, 77.418, 34.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.750
R-factor	0.179 *
Rwork	0.179
R-free	0.26300
RMSD bond length	0.016
RMSD bond angle	2.220 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *	1.860
High resolution limit [Å]	1.750	1.750
Rmerge	0.059	0.370
Number of reflections	27754 *	3703 *
Completeness [%]	94.2	77
Redundancy	6.7 *	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	PEG 1450, HEPES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 17K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG1450	28 (%)
2	1	reservoir	HEPES	100 (mM)	pH6.5