1D9U

BACTERIOPHAGE LAMBDA LYSOZYME COMPLEXED WITH A CHITOHEXASACHARIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-08-30
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	57.200, 61.081, 122.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.207 *
Rwork	0.226
R-free	0.27800 *
RMSD bond length	0.005 *
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.118	0.507
Number of reflections	14138
<I/σ(I)>	7.9
Completeness [%]	92.0	55.3
Redundancy	7.7	1.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	4.6 *	298	PEG 2000 MME, Ammonium Sulfate, pH 4.7, MICRODIALYSIS, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)
2	1	1	ammonium sulfate	0.1 (M)
3	1	1	PEG2000MME	20 (%(w/v))