1D98
THE STRUCTURE OF AN OLIGO(DA).OLIGO(DT) TRACT AND ITS BIOLOGICAL IMPLICATIONS
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Detector | HILGER-WATTS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.400, 40.700, 65.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.2 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.500 | 2.500 * |
| Number of reflections | 2572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4 * | VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.2 (mM) | |
| 2 | 1 | drop | magnesium acetate | 10.0 (mM) | |
| 3 | 1 | drop | spermine-HCl | 0.5 (mM) | |
| 4 | 1 | drop | MPD | 50 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 37.5 (%) |
