1D90
REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Detector | SIEMENS AED2 |
| Spacegroup name | P 61 |
| Unit cell lengths | 45.070, 45.070, 45.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 * - 1.700 |
| R-factor | 0.14 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.660 |
| Rmerge | 0.040 |
| Number of reflections | 3310 |
| Completeness [%] | 57.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | pH 6.50, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 2 | WATER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | octamer | 20 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 3 | 1 | drop | 100 (mM) | 0.002ml | |
| 4 | 1 | drop | water |
