1D8W
L-RHAMNOSE ISOMERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 190 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.849, 163.104, 78.150 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.174 |
| R-free | 0.24100 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 15.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.330 |
| Total number of observations | 426996 * | |
| Number of reflections | 252886 * | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 96.0 * | 84 * |
| Redundancy | 1.68 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 0.2 M CITRATE, 22.5-30% PEG 8000, 10% ISOPROPANOLE, 0.1 M HEPES, PH = 6.4 - 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | citrate | 0.2 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | |
| 6 | 1 | reservoir | PEG8000 | 22.5-30.0 (%(w/v)) | |
| 7 | 1 | reservoir | isopropanol | 10 (%(w/v)) |
