1D7Y

CRYSTAL STRUCTURE OF NADH-DEPENDENT FERREDOXIN REDUCTASE, BPHA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	288
Detector technology	DIFFRACTOMETER
Collection date	1998-05-17
Detector	WEISSENBERG
Spacegroup name	P 61 2 2
Unit cell lengths	99.200, 99.200, 175.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.100
R-factor	0.188
Rwork	0.183
R-free	0.25800 *
RMSD bond length	0.007
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.037	0.207
Total number of observations	200297 *
Number of reflections	30466 *
<I/σ(I)>	18.8
Completeness [%]	100.0 *	99.9
Redundancy	6.6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4 *	20 *	Yamada, T., (2000) Protein Pept.Lett., 7, 277. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14.0 (mg/ml)
2	1	reservoir	sodium formate	2.0 (M)
3	1	reservoir	sodium acetate	100 (mM)