1D7V
CRYSTAL STRUCTURE OF THE COMPLEX OF 2,2-DIALKYLGLYCINE DECARBOXYLASE WITH NMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 153.200, 153.200, 86.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dkb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.620 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.058 | 0.300 |
| Number of reflections | 14654 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 96.0 | 83.6 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 31.5 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 5 (mM) | |
| 3 | 1 | drop | PLP | 0.015 (mM) | |
| 4 | 1 | reservoir | sodium pyruvate | 150-200 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 15 (%) | |
| 6 | 1 | reservoir | 30 (mM) |
