1D76
CRYSTAL AND MOLECULAR STRUCTURE OF A DNA FRAGMENT CONTAINING A 2-AMINO ADENINE MODIFICATION: THE RELATIONSHIP BETWEEN CONFORMATION, PACKING, AND HYDRATION IN Z-DNA HEXAMERS
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.944, 31.282, 44.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.138 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.000 |
| High resolution limit [Å] | 1.020 |
| Number of reflections | 5790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop * | 7 | 4 * | hanging drop also O. K. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.9 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | 15 (mM) | ||
| 4 | 1 | drop | spermine tetrachloride | 5 (mM) | |
| 5 | 1 | drop | MPD | 20 (%) | |
| 6 | 1 | reservoir | MPD | 48 (%) |
