1D61
THE STRUCTURE OF THE B-DNA DECAMER C-C-A-A-C-I-T-T-G-G: MONOCLINIC FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 248 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 31.870, 25.690, 34.210 |
| Unit cell angles | 90.00, 114.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.152 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.300 |
| Total number of observations | 5848 * |
| Number of reflections | 5026 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 4 * | pH 7.10, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | CA CACODYLATE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMIDINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA decamer | 0.53 (mM) | double helix |
| 2 | 1 | drop | calcium acetate | 21.7 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 21.7 (mM) | pH7.0 |
| 4 | 1 | drop | spermidine | 0.43 (mM) | |
| 5 | 1 | drop | MPD | 9 (%) | |
| 6 | 1 | reservoir | MPD | 40 (%) |
