1D60
THE STRUCTURE OF THE B-DNA DECAMER C-C-A-A-C-I-T-T-G-G: TRIGONAL FORM
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 248 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 33.230, 33.230, 94.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.162 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.200 |
| Total number of observations | 3202 * |
| Number of reflections | 1725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA decamer | 0.53 (mM) | double helix |
| 2 | 1 | drop | magnesium acetate | 21.7 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 21.7 (mM) | pH7.0 |
| 4 | 1 | drop | spermine | 0.32 (mM) | |
| 5 | 1 | drop | MPD | 9 (%) | |
| 6 | 1 | reservoir | MPD | 35 (%) |
