1D5W
PHOSPHORYLATED FIXJ RECEIVER DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-09-19 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.150, 91.300, 59.880 |
Unit cell angles | 90.00, 112.45, 90.00 |
Refinement procedure
Resolution | 24.500 - 2.300 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 22.300 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.500 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.050 | 0.155 |
Total number of observations | 83599 * | |
Number of reflections | 26027 | |
<I/σ(I)> | 9.5 | 4.6 |
Completeness [%] | 90.0 | 93 |
Redundancy | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | drop was mixed with an equal volume of reservoir solution * |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | drop was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | EDTA | 0.1 (mM) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG1500 | 40 (%(w/v)) | |
6 | 1 | reservoir | Tris-HCl | 20 (mM) |