1D5C

CRYSTAL STRUCTURE OF PLASMODIUM FALCIPARUM RAB6 COMPLEXED WITH GDP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS
Synchrotron site	APS
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-25
Detector	MARRESEARCH
Wavelength(s)	0.9789, 0.9770, 0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	81.200, 81.200, 90.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.940 - 2.300
R-factor	0.214 *
Rwork	0.214
R-free	0.24600
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.087	0.318 *
Total number of observations	294170 *
Number of reflections	15968
<I/σ(I)>	25.4	12.6
Completeness [%]	100.0	100
Redundancy	18.4	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	sodium sulfate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium sulfate	1.0 (M)
2	1	reservoir	sodium cacodylate	0.1 (M)