1D5B
UNLIGANDED MATURE OXY-COPE CATALYTIC ANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.420, 80.670, 134.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.28500 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 26.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.280 |
| Total number of observations | 103369 * | |
| Number of reflections | 19606 * | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 91.2 * | 70.5 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 25%PEG 1000, 100mM sodium acetate, 300mM CdCl2, 100mM ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | drop | methionine | 1 (mM) | |
| 5 | 1 | reservoir | PEG1000 | 25 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | cadmium chloride | 300 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 100 (mM) |
