1D59
CRYSTAL STRUCTURE OF 4-STRANDED OXYTRICHA TELOMERIC DNA
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.730, 49.570, 97.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.990 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 |
| Total number of observations | 6322 * |
| Number of reflections | 2471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | K CACODYLATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | spermine | 6 (mM) | |
| 4 | 1 | drop | 40 (mM) | ||
| 5 | 1 | drop | potassium cacodylate | 20 (mM) | |
| 6 | 1 | drop | MPD | 5 (%) | |
| 7 | 1 | reservoir | 40 (%) |
