1D4R
29-mer fragment of human srp rna helix 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 42.980, 42.980, 231.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.570 - 2.000 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.28800 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.398 |
| Number of reflections | 17877 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | 100 MM MGCL2, 1 MM SPERMINE TETRAHYDROCHLORIDE, 50 MM MES, 15-20% (W/V) PEG 8000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | SPERMINE TETRAHYDROCHLORIDE | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | PEG 8000 | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 100 (mM) | ||
| 2 | 1 | reservoir | MES | 50 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 15-20 (%(w/v)) |
