1D4M

THE CRYSTAL STRUCTURE OF COXSACKIEVIRUS A9 TO 2.9 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1996-06
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	487.300, 358.100, 305.700
Unit cell angles	90.00, 128.10, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.169
Rwork	0.169
RMSD bond length	0.012
RMSD bond angle	1.790
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.100
High resolution limit [Å]	2.900	2.900
Rmerge	0.170
Total number of observations	697000 *
Number of reflections	290000
<I/σ(I)>	4.2
Completeness [%]	41.0	32
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.6 *		Smyth, M., (1993) J.Mol.Biol., 230, 667. *
1	Microdialysis *	7.6 *		Smyth, M., (1993) J.Mol.Biol., 230, 667. *
1	Microdialysis *	7.6 *		Smyth, M., (1993) J.Mol.Biol., 230, 667. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium dihydrogen phosphate	100 (mM)
2	1	1	sodium azide
3	1	2	ammonium sulfate	18-26 (%(v/v)sat)