1D4F
CRYSTAL STRUCTURE OF RECOMBINANT RAT-LIVER D244E MUTANT S-ADENOSYLHOMOCYSTEINE HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-27 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 91.000, 223.000, 91.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 28.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.093 * | 0.265 |
| Total number of observations | 241990 * | |
| Number of reflections | 43000 * | |
| <I/σ(I)> | 24.4 | |
| Completeness [%] | 85.6 * | 83.7 |
| Redundancy | 4.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 22 * | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | glycerol | 2 (%(v/v)) | |
| 4 | 1 | reservoir | isopropanol | 5 (%(v/v)) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
