1D4E
CRYSTAL STRUCTURE OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1 COMPLEXED WITH FUMARATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW21B |
Synchrotron site | LURE |
Beamline | DW21B |
Temperature [K] | 107 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.080, 73.080, 216.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.243 * |
Rwork | 0.243 |
R-free | 0.31100 |
RMSD bond length | 0.013 * |
RMSD bond angle | 3.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.054 |
Total number of observations | 157538 * |
Number of reflections | 15540 |
Completeness [%] | 96.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | ammonium sulfate with ethanol and fumarate added, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Bicine | 100 (mM) | |
2 | 1 | reservoir | ethanol | 1 (%(v/v)) | |
3 | 1 | reservoir | succinate | 100 (mM) | or fumarate |
4 | 1 | reservoir | ammonium sulfate | 2.6 (M) |