1D4A
CRYSTAL STRUCTURE OF HUMAN NAD[P]H-QUINONE OXIDOREDUCTASE AT 1.7 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-19 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.678, 57.032, 97.402 |
| Unit cell angles | 77.04, 76.72, 86.89 |
Refinement procedure
| Resolution | 43.630 - 1.700 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 * | 0.159 |
| Number of reflections | 98473 * | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 78.8 | 24.2 |
| Redundancy | 3.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 25 * | 30 % PEG 3350 200 MM NAACETATE 12-24 MICROM FAD 100MM NA-TRICINE PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | FAD | 0.005 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 30 (%) | |
| 5 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 6 | 1 | reservoir | sodium tricine | 100 (mg/ml) | |
| 7 | 1 | reservoir | FAD | 0.012-0.024 (mM) |
