1D49

THE STRUCTURE OF A B-DNA DECAMER WITH A CENTRAL T-A STEP: C-G-A-T-T-A-A-T-C-G

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	273
Detector technology	DIFFRACTOMETER
Detector	RIGAKU AFC-5
Spacegroup name	P 21 21 21
Unit cell lengths	38.600, 39.100, 33.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.157
Refinement software	NUCLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	1.500
Number of reflections	3793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	4 *	initial 15%(v/v) MPD reservoir whose concentration was raised 3% every week to up 40%. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	MPD
3	1	1	MG ACETATE
4	1	1	SPERMINE_HCL
5	1	2	WATER
6	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.34 (mM)
2	1	drop	magnesium acetate	0.14 (M)
3	1	drop	spermine-4HCl	0.34 (mM)
4	1	reservoir	MPD	15 (%(v/v))