1D48
STRUCTURE OF THE PURE-SPERMINE FORM OF Z-DNA (MAGNESIUM FREE) AT 1 ANGSTROM RESOLUTION
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.405, 30.768, 43.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 1.000 |
| R-factor | 0.185 |
| Refinement software | PROLSQ ((MODIFIED BY G.J.QUIGLEY)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 1.200 * |
| High resolution limit [Å] | 1.000 |
| Rmerge | 0.002 * |
| Number of reflections | 8200 |
| Completeness [%] | 35.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 2 | WATER | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.4 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 15 (mM) | |
| 4 | 1 | reservoir | 20 (%) |
